Hope, Ian A. (ed.)
C. Elegans
Chapter 7 is designed to help the user analyse phenotype by microscopy and includes Normaski, fluorescence, 4-dimensional, and electron microscopy. Techniques for studying the neurobiology of C. elegans are given in chapter 8. Chapter 9 describes the three commonly used approaches for studying gene expression and Chapter 10 deals with the common methods of molecular biology essential for gene characterization. C. elegans is not the ideal organism for biochemical studies, but chapter 11 describes several procedures for producing biochemically useful quantities of pure tissues. The final chapter is about conventional genetics and details the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. C. elegans: A Practical Approach will therefore provide all the background information necessary for use of C. elegans as a model system.
- Verlag: Oxford University Press
- Ausstattung: Zahlr. Abb. u. Tab.
- ISBN: 0199637385
- Preis: 78,95 €
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